Figure 3

Immunohistochemical labelling for the presence of OSM in vivo and in vitro. A) Section of the testis of a rat 8 days after EDS administration. All Leydig cells have disappeared from the interstitium. Under these conditions no OSM positive cells could be detected by immunohistochemistry; B) Interstitial cell were isolated from testes of 13-day-old rats and cultured for 3 or 8 days without any additions or in the presence of 10 ng/ml OSM and/or 1 ng/ml LH (for more details see Materials and Methods). Percentage of OSM positive cells in culture determined under the indicated culture conditions. The experiment was carried out in quadruplicate. Values are represented as mean ± SD; statistical analysis was carried by comparing the treated cultures with their respective controls. Means were considered to be significantly different at p < 0.05 (indicated by (a)). C) Cells cultured in the presence of LH for 8 days and immunohistochemically stained with an antibody against OSM. Some positively stained cells are indicated by arrows. Scale bar in (A) represents 21 μm, in (C) 20 μm.