Figure 2

Effect of OSM (10 ng/ml) either alone or in combination with LH (1 ng/ml) on the presence of 3β-HSD positive Leydig cell progenitors. Interstitial cells were isolated from 13-day-old rat testes and cultured for 1, 3 or 8 days. The presence of 3β-HSD was determined by enzyme histochemistry or immunohistochemistry using a polyclonal antibody against 3β-HSD (for details see Materials and Methods). Statistical analysis revealed that there was no significant difference among the different experiments and thus the results are presented as mean ± SD of three different experiments that were combined. Each single experiment was carried out either in duplicate, triplicate or quadruplicate. Differences among groups were considered to be significantly different when p < 0.05. a – significantly different from control; b – significantly different from OSM 10 ng/ml; c – significantly different from LH 1 ng/ml.