Figure 4

SP-D immunostaining of rat prostate tissue. Rats were either sham-infected or infected with E. coli. Prostates were removed 24, 48, and 72 hours post-infection. Photographs in the left column are SP-D immunostaining and the corresponding photograph on the right is the same tissue immunostained using normal rabbit IgG as a staining control. A and B. Sham-infected prostate. The rat prostate epithelium contains SP-D protein with the staining present primarily on the apical portion of the epithelial cells (arrows). C and D. Rat prostate 24 hours post-infection. The epithelial SP-D staining is present throughout the cytoplasm of the epithelial cells (arrows). E and F. Rat prostate 48 hours post-infection. Many neutrophils were present in the lumen of the prostate glands at this time. The cytoplasm of the prostate epithelium stained positive for SP-D protein (arrows) and SP-D is present in the lumen and within neutrophils as well. G and H. Rat prostate 72 hours post-infection. SP-D staining intensity remained high in some epithelial cells (arrows). Magnification bar = 100 μm.