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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Characterization of EN-1078D, a poorly differentiated human endometrial carcinoma cell line: a novel tool to study endometrial invasion in vitro

Figure 4

Sex steroid receptor status. A) Both estrogen and progesterone receptors mRNA are detected by RT-PCR but the signal for PR-A is very weak and protein is not detected by Western blot analysis. Ishikawa cell line was used as a positive control for PRA-B antibody. Data shown are representative of results obtained from three independent assays. MTT proliferation assay was conducted to verify the functionality of these receptors in EN-1078D cell line: B) 24 hours of exposure to 17β-oestradiol increases cell growth and C) a significant increase in proliferation was observed after 48 hours of exposure to 10-6M for progesterone (p < 0.05). Data shown are representative of results obtained from three independent assays.

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