A) Phase contrast features of the monolayer-cultured EN-1078D cells revealing a sheet of polygonal cells with a pavement-like arrangement. B) Transmission electron microscopy showing the tendency of cells to pile up. C) Ultrastructural aspects of EN-1078D cell line by transmission electron microscopy. Cultured cells show a high nucleus-cytoplasmic ratio, euchromatic nuclei, prominent nucleoli and well-developed microvilli. D) Higher magnification shows intracytoplasmic lipid droplets (L), and exhibits intricate cytoplasmic interdigitations but no desmosomes were observed.