Skip to main content

Table 1 Primer sequence, accession number, product size and annealing temperature of investigated genes

From: PKCepsilon and an increase in intracellular calcium concentration are necessary for PGF2alpha to inhibit LH-stimulated progesterone secretion in cultured bovine steroidogenic luteal cells

Gene

Primers

Acc#

Size

Annealing

β-Actin

F5'GACATCCGCAAGGACCTCTA3'

R5' ACGGAGTACTTGCGCTCAG3'

BC102948

100

*

PGDH

F5'GGAAAGCTGGACATCTTGGT3'

R5'GCAAATTGCGTTCAGTCTCA3'

BC102458

150

60°C

PGES-1

F 5'GAACGACCCAGATGTGGAA3'

R5'ATACGGCCCAGGAAGAAGAC3'

NM_174443

153

59°C

AKR1B5

F 5' GACCTTGGGTACCGTCACAT3'

R5'TCTTTCTCACTGGGAATCACG3'

S54973

150

59°C

PGE2 9- Reductase

F 5'AAGAAATGCAGCCGTGAACT3'

R 5' GCTCCTTCTTCTGGGCTTTT 3'

BC102943

155

59°C

COX-2

F5'CATGATGTTCTTTGTTGGCATT3'

R 5' GCGAATTCCAACTTTCCATC3'

AF031698

154

60°C

  1. • Actin was amplified under the same conditions as gene of interest