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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: PKCepsilon and an increase in intracellular calcium concentration are necessary for PGF2alpha to inhibit LH-stimulated progesterone secretion in cultured bovine steroidogenic luteal cells

Figure 2

Reduction in PKCε protein. (A) Representative Western blot showing the amount of PKCε and actin expressed in protein samples prepared from luteal steroidogenic cells after 48, 72, and 96 h of transfection with PKCε specific siRNA (lanes 1–3). Lanes labeled 4 – 6, contained protein samples from indicated control treatments (media, Non-sp siRNA, and transfection reagent, respectively). (B) Semi-quantitative analysis of the densitometry derived from four experiments similar to the one shown in panel A. the y-axis shows the ratio of the optical density ratio of PKCε to that of its corresponding β-actin. The data are shown as mean ± SEM, and comparisons were made between different treatments. Values with different letters denote differences by one-way ANOVA followed by Tukey-Kramer honestly significant difference (P < 0.05).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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