Table 1 Oligonucleotides used for RT-PCR.
Transcript | Orientation | Sequence | Nucleotide position |
|---|---|---|---|
Rxfp1 [GenBank:NM_201417] | forward | 5'-TGGAGCCCAGATTTATTCAGTGG-3' | 1722 to 1744 (exon 14) |
| Â | forward | 5'-TGTGACGAAGCCAATTTACG-3' | 337 to 356 (exon 4) |
| Â | reverse | 5'-GCCACATTTCCACCCAGATGAATG-3' | 2173 to 2150 (exon 16) |
Rxfp2 [GenBank:NM_001012475] | forward | 5'-TTCAGCGACCTTCACCTTCT-3' | 922 to 941 (exon 11) |
| Â | reverse | 5'-CTTCTGCTTGGTCGTAATGAAGTG-3' | 1696 to 1673 (exon 14) |
Mmp-7 [GenBank:NM_012864] | forward | 5'-CGGAGATGCTCACTTTGACA-3' | 603 to 622 |
| Â | reverse | 5'-TGCAAAACCCATCCACAGTA-3' | 924 to 905 |
Gapdh [GenBank:NM_017008.3] | Â | Â | Â |
| Â | forward | 5'-TGGGAAGCTGGTCATCAACGG-3' | 262 to 282 |
| Â | reverse | 5'-TGGCAGTGATGGCATGGACTG-3' | 620 to 600 |