Figure 7

[A] Relaxin fails to affect cyclic AMP production in the vas deferens. Slices of the vas deferens were incubated for 10 min with PBS (control), RLX (5 μg/ml) or FKL (50 μM) and the cyclic AMP production was measured. Results are mean ± SEM of 3 independent experiments. * P < 0.05 compared to control (Student's t test). [B] Northern blot analysis of the effect of relaxin on Mmp-7 mRNA levels in the vas deferens. Upper panel shows a representative autoradiogram with Mmp-7 and Gapdh mRNA after various periods of incubation with relaxin (1 μg/mL). Bottom panel shows densitometric analysis of two independent experiments. Error bars indicate SEM.