Figure 6

Relaxin fails to affect contractility of the vas deferens. [A] Effects of relaxin (RLX, 0.5 μg/ml) and forskolin (FKL, 50 μM) on spontaneous contractions of the vas deferens from castrated rats. [B] Effect of pre-treatment for 30 min with RLX (0.5 μg/ml) or FKL (50 μM) on contractions of the vas deferens induced by 2.5 μM noradrenaline; * P < 0.05 compared to relaxin (Student's t test). [C] Effect of RLX (0.5 μg/ml), FKL (50 μM) and IBMX (500 μM) on contractions of the vas deferens induced by incubation in a depolarizing solution; *P < 0.05 compared to relaxin (Newman Keuls). [D] Control experiment showing the relaxing effect of relaxin (RLX 0.25 μg/ml) in rat uterus. All experimental protocols were repeated at least 3 times with different animals.