Rxfp1 and Rxfp2 receptors in cultured Sertoli cells. Receptors were labeled red with immunofluorescent antibody against intracellular domains of the receptors, nuclei were labeled blue with DAPI. A and D: assay with permeabilized cells in the absence of the primary antibody. B and E: assay with non-permeabilized cells in the presence of the primary antibody. C and F: assay with permeabilized cells in the presence of the primary antibody. Rxfp1 antibody dilution 1:300, Rxfp2 antibody 1:100. Results are representative of 3 different cell cultures.