Figure 5From: Relaxin family peptide receptors Rxfp1 and Rxfp2: mapping of the mRNA and protein distribution in the reproductive tract of the male ratRxfp1 and Rxfp2 receptors in cultured Sertoli cells. Receptors were labeled red with immunofluorescent antibody against intracellular domains of the receptors, nuclei were labeled blue with DAPI. A and D: assay with permeabilized cells in the absence of the primary antibody. B and E: assay with non-permeabilized cells in the presence of the primary antibody. C and F: assay with permeabilized cells in the presence of the primary antibody. Rxfp1 antibody dilution 1:300, Rxfp2 antibody 1:100. Results are representative of 3 different cell cultures.Back to article page