Western blotting analysis of AR, P450arom, ERα and ERβ of pig sperm extracts. Individual sperm samples: lanes 1–3. LNCaP extracts were used as positive controls for AR and ERβ (lanes C+) while MCF7 extracts were used as positive controls for P450 arom and ERα (lanes C+). No bands in negative controls (lanes C-). β actin served as loading control. Molecular weights (KD) were indicated on the right of the blot.