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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: The focal adhesion protein Hic-5 is highly expressed in the rat myometrium during late pregnancy and labour and co-localizes with FAK

Figure 1

Northern blot analysis of Hic-5mRNA expression in rat myometrium during pregnancy, labour and 1 day postpartum. (A) Representative Northern blots of Hic-5 mRNA expression and 18S rRNA expression. Analysis was performed with a Hic-5-specific mouse cDNA and an 18S ribosomal rRNA-specific rabbit cDNA as templates for radiolabelled probe production. (B) Densitometric analysis illustrating the increase of Hic-5 mRNA expression during late pregnancy. Hic-5 mRNA expression on d15, d19, d21, and d23 (active labour) was significantly elevated compared to d6 (p < 0.05; *) and expression on d15, d19, and d21 was significantly elevated compared to d12 (p < 0.05; #). Furthermore, Hic-5 mRNA expression was significantly elevated on d19 and d21 (p < 0.05; **) compared to PP. Values are from 4 independent experiments (n = 4) ± SE. Days 6, 12, 15, 17, 19, 21, 22 and 23 represent gestational timepoints. NP = non-pregnant, PP = 1 day postpartum.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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