Figure 2

QPCR analysis and normalized microarray data of ANXA1 , CTDSP2 , MSX1 , HSPA1A , HSPA8 , and SULT1E1 mRNA at each stage of bovine tissue (ENDO, CAR, EEM, COT). The gene expressions on Days 25 ENDO (n = 3), 60 CAR (n = 4), 150 CAR (n = 3), 250 CAR (n = 4), 25 EEM (n = 3), 60 CAR (n = 4), 150 CAR (n = 3), and 250 CAR (n = 4) are shown. The QPCR expression of these genes was normalized to the expression of GAPDH measured in the same RNA preparation. The pink bar shows gene expression on fetal side (EEM to COT) by QPCR. The blue bar shows gene expression on maternal side (ENDO to CAR) by QPCR. The yellow line shows normalized value of the microarray. Values are means ± SEM. Values with different letters are significantly different (P < 0.05).