Figure 2
Prostaglandin F 2α and E 2 production by BEE cells over-expressing PLA2G6. Culture medium from BEE cells, transfected with PLA2G6 cDNA, was harvested 6 h after exposure to treatments and concentrations of PGs were analyzed by ELISA. Treatments included Control, C; IFNT, I; Oxytocin, O; :Oxytocin and IFNT, O&I, Bromoenol lactone, B; Oxytocin and Bromoenol lactone, B&O. Treatments were performed in triplicate and repeated (n = 6). Data are expressed as means and SEM. Columns with different superscripts are significantly different (p < 0.05). Western blot at top of figure compares PLA2G6 expression in transfected cells (lane 1) and non-transfected cells (lane 2).