Figure 2

Diameter of follicles obtained from 12-day old ICR mice and grown in in-vitro cultures for 12 days in M-199 Medium containing gonadotropins (FSH and LH 100 mIU/ml) and with the addition of Sema-4D (50 ng/ml) in the presence or absence or of specific neutralizing antibodies against Plexin-B1 (200 ng/ml). On day 9 hCG was added to the culture. Blue diamond represents the follicular diameter in the control group, Pink square represents the follicular diameter in the Sema-4D group. Turquoise triangle represents follicle diameter in the group treated with Sema-4D and neutralizing antibodies against Plexin-B1.