Figure 6

Effects of miglustat on morphology of spermatozoa from interstrain hybrid mice. Acrosomal and nuclear morphology of cauda epididymal spermatozoa from miglustat-treated (A) C57BL/6 (15 mg/kg/day) and (B) FVB/N mice (600 mg/kg/day), and from (C, D, E and F) fourth-generation C57BL/6 × FVB/N hybrid mice (15 mg/kg/day). These drug-treated hybrid mice differed in their proportion of non-falciform acrosome-less spermatozoa. This proportion increases from C to F. Acrosomes were stained with the monoclonal antibody Mab18.6 (green), and nuclei with propidium iodide (red). Bar = 10 μm.