Figure 4
PUC isolations contain a side population of verapamil-sensitive, Hoechst-dye excluding cells. A side population of Hoechstlow cells in PUC cell isolations (A) was visible after using dual-filter flow cytometry, and was partially inhibited with the addition of 50 μM verapamil (B). The percent of cells that constituted the side population was in the gated regions. A verapamil-sensitive side population was not observed in porcine fibroblasts (C and D). A summary of the side population with and without verapamil from four PUC cell isolations is in panel E.