Effects of bovine spermatozoa preparation on embryonic development in vitro

Table 1 Sperm parameters results (means ± S.E.M.)

Sperm separation protocol	Progressive motility (%)	Concentration (10⁶Ml)	HOS % active	SYBR-14/PI % live	EthD-1/FITC-PSA % live with intact acrosome
Initial (n = 6)	50.00 ± 8.16^a	82.75 ± 5.25^a	39.94 ± 8.98^a	43.34 ± 6.88^a	46.04 ± 12.56^a
BoviPure (n = 6)	70.00 ± 3.54^b	27.25 ± 1.70^b	54.35 ± 2.75^b	72.68 ± 2.79^b	75.93 ± 0.91^b
Swim up (n = 6)	53.75 ± 3.15^a	20.00 ± 5.34^b	45.90 ± 1.84^a	50.99 ± 2.18^a	59.24 ± 2.42^a

Values with different superscripts within the same columns differ significantly (P < 0.05)

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