Figure 8
PECAM-1 immunostaining in rats with increased ovarian NGF as a consequence of SON dissection. Arrows depict the predominant location of vessels in the area surrounding the ovarian follicles. Small vessels are depicted by arrowheads. A-B show the immunostaining found in control rat ovaries, and C-D show the ovaries of 6-day denervated rats, where a bigger area of capillaries and small vessels was stained. F and G show the quantification of the stained vessels area in rats denervated for 3 (n = 3) and 6 days (n = 5), respectively. The area of vessels positively stained was evaluated with the Image-J program and results were expressed as mean ± SEM. * p < 0.05 and ** p < 0.01 vs control. Original magnification 100Ă—. Bar = 100 μm.