Figure 2

mTert promoter activity in R1 ES cells. On the left, the size of the mTert-pormoter-EGFP reporter construct is shown. Cells transformations with an EGFP plasmid without promoter are used as controls for each transfection. On the right, the relative GFP expression of the three promoter construct, and the standard deviation is indicated by solid bars. The GFP intensity produced by the promoterless construct was normalized to value of 100; GFP intensity of other constructs is shown relative to this control. Results are expressed as the mean of at least three independent experiments. A, b, c indicate p < 0.05 in a one-tailed unpaired X²-test.