Figure 1

Healing and migration rate of cells were faster in multilayer wounds. A. Bright field images showing suction-wounds created in the monolayer (upper panel) and multilayer (lower panel) of cells at 0, 12, 24 and 36 h of incubation. Contrasts of images are increased to visualize the wound areas not invaded by the migrating cells. Bar Size 400 μm. B. Mean ± standard deviations of mean of wounds areas (μm²) during healing. Wound areas reduced to ~20% within 24 and 12 h of incubation in the monolayer (○) and multilayer (●) respectively. Complete healing of wound occurred earlier and by 24 h in multilayer cells. C. Mean ± standard deviations of mean of % migration rate of cells (μm²/h). Migration rate (%) in the multilayer wounds (■) remained higher until 12 h of incubation compared to those created in the mono layer (□) of cells. * p < 0.05