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Table 4 The number of eggs, percent cleavage, neurulation and swim-up during the fertile period (11.5–17.5 hrs post-induction) for Bufo baxteri having received either one or two primings. In this table, the mean number of eggs, percent cleavage, neurulation and swim-up are based only on toads that produced eggs. Data are expressed as means ± SE. A significant difference was found for egg numbers, percent neurulation and swim-up, but not for percent cleavage between the two treatment groups.

From: Hormonal priming, induction of ovulation and in-vitro fertilization of the endangered Wyoming toad (Bufo baxteri)

 

Fertilization data from one priming 11.5–17.5 hrs post-induction

Fertilization data from two primings 11.5–17.5 hrs post-induction

Toad No.

No. Eggs

Cleavage (%)

Neurulation (%)

Swim-up (%)

No. Eggs

Cleavage (%)

Neurulation (%)

Swim-up (%)

1

0

na

na

na

3,540

16.9

13.1

7.2

2

250

0

0

0

3,530

21.7

12.4

11.8

3

1,430

2

1.2

0.6

4,355

24.8

21.1

17.7

4

2,325

3.1

3.1

0

1,191

0

0

0

5

0

na

na

na

0

na

na

na

6

260

9.6

3.1

0

2,050

3.3

0

0

7

1,250

0.4

0.1

0.1

1,255

22.0

19.3

11.6

8

0

na

na

na

4,090

6.7

3.6

1.9

9

3,135

23.3

2.4

0.5

2,950

6.1

6.6

4.8

10

0

na

na

na

-

-

-

-

Mean ± SE

1,441 ± 465

6.4 ± 3.6

1.7 ± 0.6

0.2 ± 0.1

2,870 ± 436

12.7 ± 3.4

9.5 ± 2.9

6.9 ± 2.3