Table 1 List of sense (S) and antisense (AS) primers used for ZAR1 mRNA analysis.
| Â | Â | Coordinate relative to ZAR1 coding sequences and exon position | |||
|---|---|---|---|---|---|
primer | sequence (5'-3')GB acc: | bovine DQ231456 | porcine DQ231444 | human AY191416 | exon |
S1 | ccagccgagcaaggagcg | Bt (813) | Â | Â | 1 |
S2 | tacactgatggctgccctg | Bt (-8) | Â | Â | 1 |
S3 | tataacccttaccgagtggagg | Bt (976) | Â | Hs (1096) | 3 |
S4 | ctcgtaccggtacccataccc | Â | Â | Hs (60) | 1 |
S5 | acgaggtgctggacggttaca | Â | Ss (17) | Â | 1 |
S6 | cctgcgcttccagttcttaga | Bt (831) | Ss (840) | Hs (952) | 1 |
S7 | tgccgaacatgccagaag | Bt (955) | Â | Â | 1 |
S8 | aacccgttccgcgacgtat | Bt (319) | Â | Â | 1 |
S9 | tgtctcggtgcagtgctcgtt | Â | Ss (342) | Â | 1 |
S10 | aacccttatcgcgtggaggata | Â | Ss (988) | Â | 3 |
S11 | tgcccagtaaaacttcgcca | Â | Ss(1045) | Â | 4 |
AS1 | tttgaagctgaaagtgctgtcac | Bt (1143) | Ss (1152) | Hs (1263) | 4 |
AS2 | tcttcctcgccgactcctct | Â | Ss (691) | Â | 1 |
AS3 | agagggagaaaggagaagagca | Â | Ss(1261) | Â | 4 |
AS4 | gacagctttgtcaaatacagcc | Â | Ss(1307) | Â | 4 |
AS5 | acaaatcttgacggaggggcct | Bt (1090) | Â | Â | 4 |
AS6 | gaagctgaaagtgctatcacag | Bt (1140) | Â | Â | 4 |
AS7 | ggcgacgatctctcgccagcggtgtcc | Bt (803) | Â | Â | 1 |
AS8 | ataggcgtttgcctttgcatc | Bt (1117) | Ss (1124) | Â | 4 |
AS9 | ctggaagcgcaggcgctcct | Bt (843) | Â | Â | 1 |
AS10 | tcacaggataggcgtttgc | Bt (1124) | Â | Â | 4 |
AS11 | ttcacagcgggacctcagtt | Bt (1203) | Â | Â | 4 |