Figure 5
Different ZAR1 mRNA variants. (A) Detection of ZAR1 mRNAs in porcine and bovine oocytes by virtual Northern blot. Weight of molecular weight markers (kb) are indicated at the middle. (B) ZAR1 mRNA detection by classic Northern blot analysis in porcine tissues. Ethidium bromide RNA staining is at lower panels, hybridization is upper. Size of molecular weight markers (kb) are indicated in the middle. RNA from hypothalamic part of brain (br), pituitary (pi), ovaries (ov) of 7-week-old piglet and testis (te) of young boar were analysed (left gel). RNA from liver (liv), ovaries of other infantile immature (ov1) and peri-pubertal (ov2) gilts and testis of 7-month young (te1) and 2.5-year old (te2) boars were analysed. (C) Schematic representation of pig ZAR1 gene and correspondent cDNA variants, cloned from Sus scrofa oocytes (oo1, oo2, oo3) and testis (tes). The sizes of correspondent ORFs are indicated on the right. Grey bars designate exons. Chain line denoted sequences absent in tes, oo2 and oo3 cDNA variants. Palindrome repeats are indicated by arrows, their lengths are noted in brackets. The nucleotide coordinates of the beginning of palindrome repeats are marked relatively to oo1 cDNA sequence.