Figure 4

Expression ZAR1 mRNA in porcine and bovine tissues. Expression was detected by RT-PCR using S6-AS8 primers. 38 PCR cycles was performed for ZAR1, 32 and 38 cycles was performed to detect β-actin in porcine and bovine respectively. br- hypothalamus part of brain, h – heart, li – liver, pit – pituitary, i – intestine, m – muscle, lu – lung, s – spleen, ov – ovary, od – oviduct, te – testis, cu – cumulus cells, gr – granulosa cells, oo – oocyte, w – RT-PCR with water instead of RNA, RT – empty well, r – PCR with 1 μg of pooled RNA from tissues, RT omitted.