Figure 3

ZAR1 expression in oocytes and preimplantation embryos. (A) Expression ZAR1 mRNA in pig oocytes and embryos developed in vivo:GV – immature germinal vesicle oocyte, Z – presumptive zygote, 2c – 2 cells embryos, 4c – 4 cells embryos, 5-8c – 5–8 cells embryos, M – morulae, Bl – blastocyst, r – pool of embryos RNA RT omitted, w – RT with water instead of RNA. 35 and 30 PCR cycles were performed for ZAR1 and β- actin detection respectively. (B) ZAR1 mRNA expression in bovine oocytes before (GV, immature oocytes) and after in vitro maturation (MII, mature oocytes) quantified by real-time PCR. Data are presented as mean value of 3 experiments made in triplicate each +/- SEM. Difference is significant at p < 0.01.