17beta-estradiol suppresses TLR3-induced cytokine and chemokine production in endometrial epithelial cells

Table 2 Epithelial cells express TLR3 and hormone receptor mRNA.

Cell Line	TLR3	ERα	ERβ	PRA	PRB
AN3 CA	-	+	+	+	+
HEC-1-A	+	-	+	-	-
KLE	+	+	+	+	+
MCF7	+	+	+	+	+
RL95-2	+	+	+	+	+

cDNA synthesized from 100 ng RNA was subjected to end-point PCR with the indicated primer sets (see Table 1). The endometrial epithelial cell lines, AN3-CA, HEC-1-A, KLE, and RL95-2 were scored for hormone receptor expression on the basis of a visible band at the appropriate size. The breast adenocarcinoma cell line, MCF7, served as a positive control for hormone receptor expression. Cell lines were compared to the RL95-2 cells for expression of TLR3.

ISSN: 1477-7827