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Table 1 Primer couples and PCR conditions

From: Increase of mitochondrial DNA content and transcripts in early bovine embryogenesis associated with upregulation of mtTFA and NRF1 transcription factors

Gene Primer sequence D Primer sequence R PCR1 AT2 CN3
COX1 5'-AAA-TAA-TAT-AAG-CTT-CTG-ACT-CC-3' 5'-TCC-TAA-AAT-TGA-GGA-AAC-TCC-3' 190 56 4.8
mtTFA 5'-CAA-ATG-ATG-GAA-GTT-GGA-CG-3' 5'-AGC-TTC-CGG-TAT-TGA-GAC-C-3' 148 58 6.1
NRF1 5'-CCC-AAA-CTG-AGC-ACA-TGG-C-3' 5'-GTT-AAG-TAT-GTC-TGA-ATC-GTC-3' 162 58 5.6
  1. 1 PCR: PCR product length/pb
  2. 2 AT: Annealing temperature/degree C°
  3. 3 CN: Copy Number in 1 ng of PCR product × 109