Figure 6

Immunolocalisation of ghrelin and GHSR-1a in fetal sheep testes. Photomicrographs of fetal sheep testicular sections. (A, B, C, D) Sections from fetuses at day 30 of gestation showing positive immunostaining for ghrelin (A), GHSR-1a (B), SCF (C) and PCNA (D) in the fetal testis and mesonephros (meson.). (E, F, G, H) Testicular sections from fetuses at day 50 of gestation showing positive immunostaining for ghrelin (E), GHSR-1a (F), SCF (G) and PCNA (H). (I, J, K, L) Testicular sections from fetuses at day 70 of gestation showing positive immunostaining for ghrelin (I), GHSR-1a (J), SCF (K) and PCNA (L). (M, N, O, P) Testicular sections from fetuses at day 140 of gestation showing positive immunostaining for ghrelin (M), GHSR-1a (N), SCF (O) and PCNA (P) in the seminiferous cords (C) and interstitium (INT). Arrows depict germ cells. The scale bars of A, B, C, D represent 150 μm, and for the rest they represent 100 μm.