Figure 2

Immunohistochemical of ghrelin and GHSR-1a in the sheep brain. Photomicrographs of sheep brain sections. (A) Hypothalamic section including the third ventricle (3V) showing positive staining for ghrelin in the median eminence (ME), arcuate nucleus (ARC) and ventromedial hypothalamus (VMH). (B) Hypothalamic section showing positive staining for GHSR-1a in the ME, ARC and VMH. (C) High magnification micrograph showing the staining for ghrelin in the VMH. (D) High magnification micrograph showing the staining for GHSR-1a in the VMH. (E) High magnification micrograph showing the staining for ghrelin in the ARC, ME and ependymal lining (EL) of the 3V. (F) High magnification micrograph showing the staining for GHSR-1a in the ARC, ME and EL. (G) Hindbrain section including the fourth ventricle (4V) showing positive staining for ghrelin in the nucleus of the tractus solitarus (NTS). (H) Hindbrain section showing positive staining for GHSR-1a in the NTS. (I) High magnification micrograph showing the staining for ghrelin in the NTS. (J) High magnification micrograph showing the staining for GHSR-1a in the NTS. The scale bars of A, B represent 150 μm, for C, D, E, F G, H they represent 50 μm, and for I, J they represent 20 μm. The insert is the negative control.