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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

Figure 5

Melting curve (A) amplified at various annealing temparature and gel-electrophoresis pattern (B) of 18SrRNA amplified at 60° of annealing temperature by real-time PCR using the RT product of the cell lysate treated with 0.16 U/μl RNase-free DNase I for 30 min. Lane 1: 100-bp Ladder size marker; Lane 2: amplification product of 18SrRNA (120 bp) in gel-electrophoresis pattern.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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