Effects of transcriptional and translational inhibitors on oocyte maturation. Ovarian follicles were pre-treated for 2 hours with 1 mg/ml Actinomycin D, Cyclohexamide or medium (untreated) and then incubated with: (A) Medium only (control), TGF-β1 (10 ng/ml), hCG (100 IU/ml), or a combination of hCG and TGF-β1 or (B) Medium only (control), TGF-β1 (10 ng/ ml), MIH (100 ng/ml) or a combination of MIH and TGF-β1 for 18 hours. The rate of maturation was scored as percentage of follicles that underwent GVBD. Data represent the mean ± SEM of one representative experiment with four replicates. Different letters denote statistical significance (P < 0.05).