Figure 8
Confocal scanning laser micrographs showing distribution of PAD in in vivo fertilized oocytes and in vivo matured pre-implantation embryos labeled with anti-PAD V (N). (A – C) Three different focal sections of a zona free fertilized oocyte showing PAD labeling on the oocyte's surface (arrowhead) and in both pronuclei (arrows). (D, E) A 2-cell embryo and an 8-cell embryo showed PAD labeling on the blastomeres' surfaces (arrowhead) and in their nuclei (arrows). (F) A blastocyst showing that anti-PAD V (N) labeled the trophoblast cells, and inner cell mass cells, and nuclei of inner cell mass cells (arrow).