Figure 4

Effect of estradiol benzoate or estradiol benzoate plus bromoergocryptine on DNA fragmentation in CL incubated in serum-free conditions. At the end of the incubation, genomic DNA was extracted from vehicle-treated, estradiol benzoate (EB)-treated or EB plus bromoergrocriptine (BEC)-treated animals and run on a gel (Panel A). Densitometry of the DNA fragments studied from three different experiments with similar outcome is also shown (Panel B). * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with values at time zero (one way analysis of variance followed by the Dunnett multiple-comparison test). † P < 0.05 and †† P < 0.01 compared with the corresponding time across treatments (one way analysis of variance followed by the Tukey multiple comparison test). bp, Base pairs. Only CL of previous pregnancy were evaluated.