Table 2 Description of the primers used for RT-PCR. The sequences, the number of cycles, annealing temperature (Tm) and product size of primers used for RT-PCR analysis.
Genes | 5' Primer | 3' Primer | Cycles | Tm °C | Size bp |
|---|---|---|---|---|---|
IL-8 | TCTCTTGGCAGCCTTCCT | AATTCTCAGCCTCTTCAAAAACTT | 34 | 61 | 276 |
MCP-1 | CTCTGCCGCCCTTCTGTGCC | GTCTTCGGAGTTTGGGTTTGC | 28 | 61 | 288 |
RANTES | CGGCACGCCTCGCTGTCATC | TGTACTCCCGAACCCATTT | 34 | 61 | 240 |
28S | GTGCAGATCTTGGTGGTAGTAGC | AGAGCCAATCCTTATCCCGAAGTT | 19 | 58 | 552 |