Figure 7
Representative saturation curves and Scatchard analyses. Saturation curves describing [3H]-DHT binding to AR in cytosolic fraction (A), [3H]-KT binding in cytosolic fraction (C), [3H]-DHT binding to AR in nuclear fraction (E) and [3H]-DHT binding to AR in reticulocyte lysate samples (G). Specific binding (â–¼) was determined by subtracting non-specific binding (â– ) from total binding (â–²). Scatchard analyses of the specific binding of [3H]-DHT to cytosolic fraction (B), [3H]-KT to cytosolic fraction (D), of [3H]-DHT to nuclear fraction (F) and of [3H]-DHT to reticulocyte samples (H).