Interferon-gamma alters the phagocytic activity of the mouse trophoblast

Table 1 Erythrophagocytic activity of cultured F1-trophoblast giant cells treated with leukocyte conditioned medium and mrIFN-γ

Culture treatment	n	PI (%)
Standard culture medium (SCM)	5	5.1 ± 0.95
SCM + ^AbIFN-γ	3	7.14 ± 1.93
SCM + ^AbC	4	6.44 ± 3,34
Leukocyte conditioned medium (LCM)	7	38.95 ± 3.50*
LCM+^AbIFN-γ	4	18.55 ± 0.22 /*
mrIFN-γ 100 U/mL	3	34.99 ± 1.40/**

Values significantly different at p < 0.001: *compared to the control containing only standard culture medium and erythrocytes (SCM); ** compared to treatment with leukocyte conditioned medium (LCM) and, *** compared to treatment with LCM (200 μL/mL) plus ^AbIFN-γ. PI, phagocytic index calculated as the percentage of trophoblast giant cells containing erythrophagosomes. ^AbIFN-γ, monoclonal antibody XMG 1.2 against IFN-γ (200 μg/mL). ^AbC, isotype matched control antibody, (200 μg/mL). mrIFN-γ, mouse recombinant IFN-γ (100 U/mL).

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