Table 1 Description of primers and probes used for Real-time RT-PCR. Accession number (Entrez Nucleotide Database), sequences of primers and probes for human iNOS, eNOS, and bNOS; optimal primer and probe concentrations; number of PCR-cycles.
From: mRNA expression and localization of bNOS, eNOS and iNOS in human cervix at preterm and term labour
Target | Accession # | Type | Sequence | Concentration (nM) | N cycles |
|---|---|---|---|---|---|
iNOS | XM_034166 | FWD | TGGATGCAACCCCATTGTC | 300 | 60 |
REV | CCCGCTGCCCCAGTTT | 50 | |||
Probe | 6FAM-TCCCCACGGCATGTGAGGATCA-TAMRA | 200 | |||
eNOS | AF400594 | FWD | CGGCATCACCAGGAAGAAGA | 300 | 50 |
REV | CATGAGCGAGGCGGAGAT | 300 | |||
Probe | 6FAM-TTCACGGCGTTGGCCACTTCTTTAAA-TAMRA | 200 | |||
bNOS | NM_000620 | FWD | GGATCACATGTTCGGTGTTCAG | 300 | 50 |
REV | CCCAACTTTGCGCTTGAAGA | 900 | |||
Probe | 6FAM-AAATCCAGCCCAATGTCATTTCTGTTCG-TAMRA | 200 |