Figure 1

Detection of apoptosis in pregnant endometrial tissues as demonstrated by Western and TUNEL analyses. A) Apoptosis as determined by Western analysis of cleaved caspase-3 (one blot presented out of 6). β-actin blots shown were used as controls to correct for loading in each lane. Blots shown are from one representative experiment. Graphics represent Western blot densitometrical analysis of the 17 KDa cleaved fragment. B) Cleaved caspase-3 immunofluorescence at day 14 of pregnancy. Magnification: control: 10× ; inset and Day 14: 40×. C) TUNEL analysis at day 14 of pregnancy. B) and C) One representative section is presented out of 6 experiments. C-: negative control. Magnification: control: 10× ; inset and Day 14: 40×. Arrows indicate positive staining.