Effect of anti-α6 IgG on cell numbers, pyknotic rates and steroidogenesis of GC from large follicles cultured on substrata containing different ratios of LN and RGD peptides. GC from large follicles were cultured for 144 h as described in legend of Figure 5. (a): numbers of cells at 144 h of culture; (b): pyknotic rates of GC at 48 h of culture. (c) and (d): estradiol and progesterone secretions between 96 h and 144 h of culture. Empty bars: control; solid bars: with anti-α6 IgG in culture medium. Data represent mean ± SEM of 6 to 8 independent experiments. * : p < 0.05, ** : p < 0.01, *** : p < 0.001, with vs. without anti-α6 IgG.