(Top panel) Western blotting of rat Sertoli cell lysates treated with polyclonal antibodies raised against clusterin. Representative data of n = 3 independent experiments. (Lower panel) Quantification of immunoblot by densitometry. The amount of 40-kDa was quantified by integrating the volume as described under "Methods". Results are expressed as mean +/- SEM of three determinations. 1. control; 2. MAA; 3. MAA plus ω-agatoxin (p < 0.001).