Figure 2

Protein content, cytochrome c oxidase activity, and HSD activity in total homogenates, and in mitochondrial and microsomal fractions from ovaries of C31 mice that were pregnant for 5 days, 10 days, 15 days, and 20 days, or were 5 days postpartum. Each experimental group consisted of 6 mice. Mitochondrial and microsomal fractions were isolated by differential centrifugation. Results of two separate experiments are expressed per individual ovary. Statistical analyses of the enzymatic activities of cytochrome c oxidase and HSD in total homogenates at each time-point showed a significant difference @ P < .05, when compared to animals in diestrus. In addition, the HSD activities in all mitochondrial and microsomal fractions of pregnant mice were significantly different @ P < .05, when compared to animals in diestrus.