Table 3 In vitro fertilization of E. coqui eggs
Fertilization protocol | Number that developed to neurula/Number tested (%) |
|---|---|
Sperm solution dripped over the eggs | 5/63 (8) |
Testes minced directly over eggs | 8/30 (27) |
Sperm solution injected under the jelly coat | 0/12 |
Sperm incubated in jelly buffer and then injected under the jelly coat | 1/10 (10) |
Poked, then fertilized 15 minutes later by mincing testes directly over the eggs | 0/36 |