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Table 3 In vitro fertilization of E. coqui eggs

From: In vitro fertilization and artificial activation of eggs of the direct-developing anuran Eleutherodactylus coqui

Fertilization protocol Number that developed to neurula/Number tested (%)
Sperm solution dripped over the eggs 5/63 (8)
Testes minced directly over eggs 8/30 (27)
Sperm solution injected under the jelly coat 0/12
Sperm incubated in jelly buffer and then injected under the jelly coat 1/10 (10)
Poked, then fertilized 15 minutes later by mincing testes directly over the eggs 0/36