Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

Table 3 Effect of eFSH concentration on nuclear maturation and cumulus expansion.

Maturation medium	Nuclear maturation rate % (n° of oocytes)	Cumulus expansion rate % (n° of oocytes)
Control	50% (28) ^a	33% (15) ^a
Control + 0.1 μg/ml eFSH	27% (26) ^a	36% (14) ^a
Control + 0.5 μg/ml eFSH	37% (27) ^a	27% (15) ^a
Control + 1 μg/ml eFSH	37% (27) ^a	47% (15) ^a
Control + 5 μg/ml eFSH	50% (28) ^a	43% (14) ^a
Control without BSA with 20% FCS	50% (26) ^a	100% (14) ^b

ISSN: 1477-7827