Figure 6
No induction of iNOS mRNA expression in human fetal membranes (FM), cultured without serum, following treatment with PAF (10 nM). Lane 1 fetal membranes immediately after collection, lane 2 control, lane 3 = 10 nM PAF, lane 4 positive control, lanes M ΦX174 DNA/Hae III marker. This is one example of four identical results with four different samples of fetal membranes. The fetal membrane samples were initially negative for iNOS mRNA, as detected by the standard RT-PCR method.