Reproductive Biology and Endocrinology

Table 3 Transcript expression

From: Expression of connexins in human preimplantation embryos in vitro

	PN			2 cell			4 cell			8 cell			Blastocyst
Gene	1	2	3	1	2	3	1	2	3	1	2	3	1	2	3
β actin	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Cx26	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-
Cx31	+	+	-	-	+	+	+	+	+	+	+	-	+	-	+
Cx32	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
Cx40	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
Cx43	+	+	+	+	-	+	-	+	-	+	+	+	+	+	+
Cx45	-	+	-	+	-	-	-	+	-	-	-	-	-	-	-

Expression of β actin, connexins 26, 31, 32, 40, 43 and 45 in cDNAs amplified from three individual embryos (1,2,3) at the pronucleate (PN), 2-cell, 4-cell, 8-cell, and blastocyst stages of development. + denotes trancript detected, – denotes transcript not detected.

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