Figure 5
Estrous cycle (A) and pregnancy (B) effects on osteopontin levels inbovine endometrium. Tissue sections from stages 1 (metestrus), 2 (early diestrus), 3 (late diestrus), and 4 (proestrus/estrus) of the estrous cycle and from early pregnancy (days 16, 18, 21, 24 and 30) were scored by two independent observers for staining intensity of the luminal epithelium, superficial glands and deep glands Osteopontin reactivity in luminal epithelium decreased in late diestrus. As pregnancy progressed, apical cell reactivity to osteopontin antibody decreased in the glands. * means significantly different from other stages (P < 0.05).