Fundulus heteroclitus gonadotropins.5: Small scale chromatographic fractionation of pituitary extracts into components with different steroidogenic activities using homologous bioassays

Table 1 Maturation-inducing and steroidogenic activities in active fractions derived from a combination of chromatofocusing followed by HIC^a

^aResults presented as means ± SEM derived from three chromatographic series. In the absence of added gonadotropin, no GVBD took place and T- and DHP-production were undetectable, while E₂-production averaged 1,150 pg per well. The latter baseline value was subtracted from the induced E₂ data to provide the indicated values for E₂. ^bThawed, immediately added to L-15 medium, and tested for activity at a concentration of 0.5 pit. equiv./well (0.25 pit. equiv./ml). ^cThawed, stored for up to a week at 4°C, dialyzed overnight against distilled water, dried by vacuum centrifugation, reconstituted with L-15 medium, and tested for activity at a concentration of 0.5 pit. equiv./well. ^dRoman numerals refer to portions of the chromatofocusing runs (Fig. 4) while Arabic numerals refer to active fractions found after subsequent HIC (Fig. 5).

ISSN: 1477-7827