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Table 1 Maturation-inducing and steroidogenic activities in active fractions derived from a combination of chromatofocusing followed by HICa

From: Fundulus heteroclitus gonadotropins.5: Small scale chromatographic fractionation of pituitary extracts into components with different steroidogenic activities using homologous bioassays

   pg per well X 100
Sample GVBD (%) E2 T DHP T/E2 DHP/E2
FPE b 38 ± 7 5,850 ± 2,000 264 ± 48 332 ± 68 4.5 5.7
FPE c 40 ± 5 4,450 ± 2,000 240 ± 56 312 ± 48 5.4 7.0
I-1 d 12 ± 12 3,150 ± 1,204 147 ± 53 126 ± 18 4.7 4.0
II-8 d 29 ± 8 3,300 ± 450 145 ± 14 139 ± 49 4.4 4.2
III-10 d 4 ± 3 2,120 ± 701 60 ± 60 43 ± 15 2.8 2.0
V-19 d 26 ± 15 4,483 ± 393 271 ± 23 339 ± 91 6.0 7.6
  1. aResults presented as means ± SEM derived from three chromatographic series. In the absence of added gonadotropin, no GVBD took place and T- and DHP-production were undetectable, while E2-production averaged 1,150 pg per well. The latter baseline value was subtracted from the induced E2 data to provide the indicated values for E2. bThawed, immediately added to L-15 medium, and tested for activity at a concentration of 0.5 pit. equiv./well (0.25 pit. equiv./ml). cThawed, stored for up to a week at 4°C, dialyzed overnight against distilled water, dried by vacuum centrifugation, reconstituted with L-15 medium, and tested for activity at a concentration of 0.5 pit. equiv./well. dRoman numerals refer to portions of the chromatofocusing runs (Fig. 4) while Arabic numerals refer to active fractions found after subsequent HIC (Fig. 5).