Figure 3
From: Stimulation of TM3 Leydig cell proliferation via GABAA receptors: A new role for testicular GABA
PCNA in postnatal and adult rat testis. Immunohistochemical testicular localization of the proliferation marker PCNA (proliferating cell nuclear antigen) revealed that germ cells and Sertoli cells are immunopositive for PCNA, as seen in adult rat testis (A) and five days old (5d) rat testis (B). In interstitial spaces of adult testis (A) we sporadically found immunopositive cells (insert panel, A). In postnatal testis (B) abundant immunoreactive cells (*) were identified in the interstitium. No reaction was observed in sections incubated with buffer or non-immune serum (C, D) instead of the primary antibody. Bars: 25 μm.